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71.
Aline S. Turque Alexander M. Cardoso Cynthia B. Silveira Ricardo P. Vieira Flávia A. D. Freitas Rodolpho M. Albano Alessandra M. Gonzalez Rodolfo Paranhos Guilherme Muricy Orlando B. Martins 《Marine Biology》2008,155(2):135-146
In this study we performed a survey of the bacterial communities associated with the Western Atlantic demosponges Hymeniacidon heliophila and Polymastia janeirensis, based on 16S rRNA sequencing and transmission electron microscopy (TEM). We compared diversity and composition of the sponge-associated
bacteria to those of environmental bacteria, represented by free-living bacterioplankton and by bacteria attached to organic
particulate matter in superficial sediments. Partial bacterial 16S rRNA sequences from seawater, sediment, and sponges were
retrieved by PCR, cloning, and sequencing. Sequences were subjected to rarefaction analyses, phylogenetic tree construction,
and LIBSHUFF quantitative statistics to verify coverage and similarity between libraries. Community structure of the free-living
bacterioplankton was phylogenetically different from that of the sponge-associated bacterial assemblages. On the other hand,
some sediment-attached bacteria were also found in the sponge bacterial community, indicating that sponges may incorporate
bacteria together with sediment particles. Rare and few prokaryotic morphotypes were found in TEM analyses of sponge mesohyl
matrix of both species. Molecular data indicate that bacterial richness and diversity decreases from bacterioplankton, to
particulate organic sediment, and to H. heliophila and P. janeirensis. Sponges from Rio de Janeiro harbor a pool of novel and exclusive sponge-associated bacterial taxa. Sponge-associated bacterial
communities are composed of both taxons shared by many sponge groups and by species-specific bacteria. 相似文献
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Tânia Vieira Madureira Susana Velhote Cláudia Santos Catarina Cruzeiro Eduardo Rocha Maria João Rocha 《Environmental science and pollution research international》2014,21(9):6089-6098
A new and fully validated QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction and gas chromatography–tandem mass spectrometry methodology was developed and subsequently implemented for the quantification of 16 polycyclic aromatic hydrocarbons (PAHs) in wild (from Matosinhos Beach, Portugal) and commercial (from Ria de Arousa, Spain) mussels. The method proved to be robust, precise, and accurate, with recoveries ranging from 89.2 to 111.8 %. Total sums of 16 PAHs were 52.91 and 37.58 ng/g of wet weight for wild and commercial specimens, respectively. The three- to four-ring PAHs were the most abundant, and a mixture of petrogenic and pyrolytic sources were suspected to occur in both origin areas. Although the contamination levels were below the European regulated limits, specifically for commercial mussels (this despite wild specimens are also consumed), care should be taken in terms of human health, since we are still not aware of the low-dose versus long-term effects, even more in high-risk population groups. 相似文献
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Paíga Paula Sousa Sara Vera José Bitencourt Luciana Vieira Joana Jorge Sandra Silva Jaime Gabriel Correia Manuela Domingues Valentina F. Delerue-Matos Cristina 《Environmental science and pollution research international》2021,28(47):66787-66803
Environmental Science and Pollution Research - Three sampling campaigns were performed in the Lis River (Leiria, Portugal) in February of 2018, November of 2018, and May of 2019. River water and... 相似文献
79.
Ana Luiza Machado Terra Roberta da Costa Kosinski Juliana Botelho Moreira Jorge Alberto Vieira Costa 《Journal of environmental science and health. Part. B》2013,48(8):709-716
The occurrence of diseases in cultivars has caused significant losses in global food production. The advancement of nanobiotechnology makes it possible to obtain new products to be used in the control of pathogens in cultivars. Silver nanoparticles can be synthesized by microalgae and are widely known for their antimicrobial activity. In addition, the biomass produced in microalgal culture for the biosynthesis of the nanoparticles also demonstrates antimicrobial properties, as it can increase the antibacterial and antifungal potential of the silver nanoparticles. In this context, this article addresses the use of microalgae to biosynthesize silver nanoparticles simultaneously with biomass production. In addition, we demonstrate the antimicrobial potential of these nanomaterials, as well as of the microalgal biomass produced in biosynthesis, to use in the control of pathogens in agriculture. 相似文献
80.
Judite Vieira André Fonseca Vítor J. P. Vilar Rui A. R. Boaventura Cidália M. S. Botelho 《Environmental science and pollution research international》2013,20(1):508-524
The aim of the study was to predict the impact of flow conditions, discharges and tributaries on the water quality of Lis River using QUAL2Kw model. Calibration of the model was performed, based on data obtained in field surveys carried out in July 2004 and November 2006. Generally the model fitted quite well the experimental data. The results indicated a decrease of water quality in the downstream area of Lis River, after the confluence of Lena, Milagres and Amor tributaries, as a result of discharges of wastewaters containing degradable organics, nutrients and pathogenic organisms from cattle-raising wastewaters, domestic effluents and agricultural runoff. The water quality criteria were exceeded in these areas for dissolved oxygen, biochemical oxygen demand, total nitrogen and faecal coliforms. Water quality modelling in different scenarios showed that the impact of tributaries on the quality of Lis River water was quite negligible and mainly depends on discharges, which are responsible by an increase of almost 45, 13 and 44 % of ultimate carbonaceous biochemical oxygen demand (CBODu), ammonium nitrogen and faecal coliforms, for winter simulation, and 23, 33 and 36 % for summer simulation, respectively, when compared to the real case scenario. 相似文献